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Title: Multistep microreactions with proteins using electrocapture technology
Authors: Astorga-Wells, Juan
Bergman, Tomas
Jörnvall, Hans
Subjects: FRASCATI::Natural sciences
Subjects MESH: Animals
Peptide Mapping
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Issue Date: 1-May-2004
Source: Analytical chemistry
Volume: 76
Issue: 9
First Page: 2425-9
Last Page: 2429
Abstract: A method to perform multistep reactions by means of electroimmobilization of a target molecule in a microflow stream is presented. A target protein is captured by the opposing effects between the hydrodynamic and electric forces, after which another medium is injected into the system. The second medium carries enzymes or other reagents, which are brought into contact with the target protein and react. The immobilization is reversed by disconnecting the electric field, upon which products are collected at the outlet of the device for analysis. On-line reduction, alkylation, and trypsin digestion of proteins is demonstrated and was monitored by MALDI mass spectrometry.
ISSN: 0003-2700
Other Identifiers: 10.1021/ac0354342
Appears in Collections:Master in Music & Society

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